BAX antibody (AA 1-192)

Catalog No. ABIN5700359

The Rabbit Polyclonal anti-BAX antibody has been validated for WB, IHC, ELISA, IF, FACS and IP. It is suitable to detect BAX in samples from Human, Mouse, Rat, Rabbit and Sheep.

Quick Overview for BAX antibody (AA 1-192) (ABIN5700359)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-BAX Antibody

Binding Specificity: AA 1-192

Purpose: BAX antibody

Immunogen:

Immunogen sequence: 1-192aa

Immunogen: BCL2-associated X protein

Isotype: IgG

Application Details

Application Notes: WB : 1:1000-1:10000 IP : 1:1000-1:10000 IHC : 1:20-1:200 IF : 1:10-1:100

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00810(BAX antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX

Background:

Synonyms: Apoptosis regulator BAX|Bcl-2-like protein 4 (Bcl2-L-4)|BAX|BCL2L4

Background: BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction(PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria(PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis(PMID: 8183579).

Molecular Weight: 21 kDa

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response