ERK1 antibody

Catalog No. ABIN5702776

This Rabbit Polyclonal antibody specifically detects ERK1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Zebrafish (Danio rerio).

Quick Overview for ERK1 antibody (ABIN5702776)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-ERK1 Antibody

Purpose: ERK1/2 antibody

Immunogen: mitogen-activated protein kinase 1

Isotype: IgG

Application Details

Application Notes: WB : 1:1000-1:10000 IHC : 1:50-1:200 IF : 1:10-1:100

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab02845(ERK1/2 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1

Background:

Synonyms: Mitogen-activated protein kinase 1 (MAP kinase 1, MAPK 1)|ERT1|Extracellular signal-regulated kinase 2 (ERK-2)|MAP kinase isoform p42 (p42-MAPK)|Mitogen-activated protein kinase 2 (MAP kinase 2, MAPK 2)|MAPK1|ERK2|PRKM1|PRKM2

Background: This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Molecular Weight: 38-44 kDa

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins