RENT1/UPF1 antibody

Catalog No. ABIN5704268

The Rabbit Polyclonal anti-RENT1/UPF1 antibody has been validated for WB, IHC, ELISA, IF and IP. It is suitable to detect RENT1/UPF1 in samples from Human, Mouse and Rat.

Quick Overview for RENT1/UPF1 antibody (ABIN5704268)

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RENT1/UPF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RENT1/UPF1 Antibody

Immunogen: UPF1 regulator of nonsense transcripts homolog (yeast)

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000,IHC:1:20-1:200,IF:1:20-1:200,IP:1:200-1:2000

Comment:

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09270(UPF1 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Alternative Name: UPF1

Background: Synonyms:ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1 Background:This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.

Molecular Weight: 43kDa

Gene ID: 5976

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome