Hepcidin antibody (N-Term)

Catalog No. ABIN570711

This anti-Hepcidin antibody is a Goat Polyclonal antibody detecting Hepcidin in IHC and ELISA. Suitable for Human.

Quick Overview for Hepcidin antibody (N-Term) (ABIN570711)

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Hepcidin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Hepcidin Antibody

Binding Specificity: N-Term

Purpose: HAMP

Sequence: TSGSVFPQQT GQLAE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TSGSVFPQQTGQLAE-C, from the N Terminus (near) of the protein sequence according to NP_066998.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Liver lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.41k

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Hepcidin

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: HAMP

Background: HAMP, hepcidin antimicrobial peptide, HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide, putative liver tumor regressor

Gene ID: 57817

NCBI Accession: NP_066998

Pathways: Hormone Activity, Transition Metal Ion Homeostasis