TRPC7 antibody (Internal Region)

Catalog No. ABIN570738

Cited in 2+ publications. The Goat Polyclonal anti-TRPC7 antibody (ABIN570738) specifically detects TRPC7 in WB, IP and ELISA. The antibody is reactive with Mouse and Chicken samples.

Quick Overview for TRPC7 antibody (Internal Region) (ABIN570738)

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Reactivity: Mouse, Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPC7 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Grade: Verified

Product Details anti-TRPC7 Antibody

Binding Specificity: Internal Region

Purpose: TRPC7

Sequence: KLCKSKAKSC END

Specificity: This antibody is expected to cross-react with isoform 1, 2 and 3 (NP_065122.1, NP_001161049.1, NP_001161048.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KLCKSKAKSCEND, from the internal region of the protein sequence according to NP_065122.1, NP_001161049.1, NP_001161048.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: A 95 kDa band was observed in Chicken Embryo Heart atrial and ventricular myocyte lysates (Sabourin et al, PMID 21672930). This antibody has been successfully used in WB on Mouse (Murtaza et al., PMID: 32882106).

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation: This antibody has been successfully used in IP on lysates of Chicken Embryo Heart (Sabourin et al, PMID: 21672930).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TRPC7 antibody (ABIN570738)

Sabourin, Antigny, Robin, Frieden, Raddatz: "Activation of transient receptor potential canonical 3 (TRPC3)-mediated Ca2+ entry by A1 adenosine receptor in cardiomyocytes disturbs atrioventricular conduction." in: The Journal of biological chemistry, Vol. 287, Issue 32, pp. 26688-701, (2012) (PubMed).

Sabourin, Robin, Raddatz: "A key role of TRPC channels in the regulation of electromechanical activity of the developing heart." in: Cardiovascular research, Vol. 92, Issue 2, pp. 226-36, (2011) (PubMed).

Target Details for TRPC7

Target: TRPC7 (Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7))

Alternative Name: TRPC7

Background: TRPC7, transient receptor potential cation channel, subfamily C, member 7, TRP7, likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7, putative capacitative calcium channel

Gene ID: 57113, 26946, 282822

NCBI Accession: NP_065122, NP_001161049, NP_001161048