Insulin antibody

Catalog No. ABIN5707536

The Rabbit Monoclonal anti-Insulin antibody has been validated for IHC (p). It is suitable to detect Insulin in samples from Human, Pig, Mouse, Cow and Rabbit.

Quick Overview for Insulin antibody (ABIN5707536)

Target: Insulin (INS)

Reactivity: Human, Pig, Mouse, Cow, Rabbit

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IRDN-1980R

Product Details anti-Insulin Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Purified pig insulin conjugated to KLH was used as the immunogen for the recombinant INS antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant INS antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant INS antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation