NGFR antibody
Quick Overview for NGFR antibody (ABIN5707549)
Target
See all NGFR AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Cross-Reactivity (Details)
- Not suitable for mouse
-
Purification
- Purified
-
Purity
- Protein A affinity chromatography
-
Immunogen
- Recombinant human protein was used as the immunogen for the recombinant p75NTR antibody.
-
Isotype
- IgG kappa
-
-
-
-
Application Notes
-
Optimal dilution of the recombinant p75NTR antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
-
Restrictions
- For Research Use only
-
-
-
Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store the recombinant p75NTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
-
-
- NGFR (Nerve Growth Factor Receptor (NGFR))
-
Alternative Name
- NGF Receptor / p75NTR / CD271
-
Background
- This antibody is specific for a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or p75 Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR/p75NTR is a reliable marker for desmoplastic and neurotropic melanomas. It is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR/p75NTR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
-
Pathways
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
Target
-