Recombinant CD43 antibody

Catalog No. ABIN5707565

This Rabbit Monoclonal antibody specifically detects CD43 in FACS, WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant CD43 antibody (ABIN5707565)

Target: CD43 (SPN) (Sialophorin (SPN))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPN-2049R

Product Details anti-CD43 Antibody

Purpose: Recombinant CD43 Antibody / Rabbit Monoclonal

Purification: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant CD43 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity