Recombinant CD43 antibody

Catalog No. ABIN5707567

The Rabbit Monoclonal anti-CD43 antibody (Clone SPN-2049R) (ABIN5707567) specifically detects CD43 in FACS, WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant CD43 antibody (ABIN5707567)

Target: CD43 (SPN) (Sialophorin (SPN))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPN-2049R

Product Details anti-CD43 Antibody

Purpose: Recombinant CD43 Antibody / Rabbit Monoclonal (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant CD43 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity