GFAP antibody

Catalog No. ABIN5707862

This Rabbit Monoclonal antibody specifically detects GFAP in IHC (p). It exhibits reactivity toward Human, Rat, Mouse and Pig.

Quick Overview for GFAP antibody (ABIN5707862)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ASTRO-1974R

Product Details anti-GFAP Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.

Isotype: IgG kappa

Application Details

Application Notes: The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP / Glial Fibrillary Acidic Protein

Background: This mAb recognizes a protein of ~50  kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.