SOX10 antibody (AA 115-269)

Catalog No. ABIN5707896

The Rabbit Monoclonal anti-SOX10 antibody has been validated for IHC (p). It is suitable to detect SOX10 in samples from Human.

Quick Overview for SOX10 antibody (AA 115-269) (ABIN5707896)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SOX10-2311R

Product Details anti-SOX10 Antibody

Binding Specificity: AA 115-269

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Amino acids 115-269 of the human protein were used as the immunogen for the recombinant SOX10 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant SOX10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant SOX10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Pathways: Chromatin Binding