CD14 antibody (AA 16-260)

Catalog No. ABIN5708067

The Rabbit Polyclonal anti-CD14 antibody has been validated for WB, IHC (p) and ELISA (Capture). It is suitable to detect CD14 in samples from Mouse and Rat.

Quick Overview for CD14 antibody (AA 16-260) (ABIN5708067)

Target: CD14

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA (Capture)

Product Details anti-CD14 Antibody

Binding Specificity: AA 16-260

Purification: Antigen affinity purified

Immunogen: A recombinant mouse protein corresponding to amino acids S16-A260 was used as the immunogen for the CD14 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD14 antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA (Capture, recombinant mouse protein): 0.1-0.5 μg/mL (BSA-free formulation available)

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55 kDa glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades