OXT antibody (C-Term)

Catalog No. ABIN570836

The Goat Polyclonal anti-OXT antibody has been validated for ELISA, IF and FACS. It is suitable to detect OXT in samples from Mouse. There is 1 publication available.

Quick Overview for OXT antibody (C-Term) (ABIN570836)

Target: OXT (Oxytocin, Prepropeptide (OXT))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This OXT antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-OXT Antibody

Binding Specificity: C-Term

Purpose: OXT (mouse)

Sequence: DGCRTDPACD PES

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DGCRTDPACDPES

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 22 kDa band in Mouse Liver after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9 kDa according to NP

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-OXT antibody (ABIN570836)

McMahon, Chang, Jackson: "Multiple cytosolic calcium buffers in posterior pituitary nerve terminals." in: The Journal of general physiology, Vol. 147, Issue 3, pp. 243-54, (2016) (PubMed).

Target Details for OXT

Target: OXT (Oxytocin, Prepropeptide (OXT))

Alternative Name: Oxt

Background: Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535

Gene ID: 18429, 25504

NCBI Accession: NP_035155

Pathways: Myometrial Relaxation and Contraction, Feeding Behaviour