SLC18A2 antibody (C-Term)

Catalog No. ABIN570915

Cited in 2+ publications. The Goat Polyclonal anti-SLC18A2 antibody (ABIN570915) specifically detects SLC18A2 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for SLC18A2 antibody (C-Term) (ABIN570915)

Target: SLC18A2 (Solute Carrier Family 18 (Vesicular Monoamine Transporter), Member 2 (SLC18A2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC18A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SLC18A2 Antibody

Binding Specificity: C-Term

Purpose: VMAT2 / SLC18A2

Sequence: SYPIGEDEES ESD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SYPIGEDEESESD, from the C Terminus of the protein sequence according to NP_003045.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5-6 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Adrenal Gland lysates (calculated MW of 55.7 kDa according to NP_003045.2). Recommended concentration 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SLC18A2 antibody (ABIN570915)

Zhang, Morales: "Ultrastructural Detection of Neuronal Markers, Receptors, and Vesicular Transporters." in: Current protocols in neuroscience, Vol. 88, Issue 1, pp. e70, (2020) (PubMed).

Zhang, Qi, Li, Wang, Britt, Hoffman, Bonci, Lupica, Morales: "Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons." in: Nature neuroscience, Vol. 18, Issue 3, pp. 386-92, (2015) (PubMed).

Target Details for SLC18A2

Target: SLC18A2 (Solute Carrier Family 18 (Vesicular Monoamine Transporter), Member 2 (SLC18A2))

Alternative Name: SLC18A2

Background: SLC18A2, VMAT2, SVAT, SVMT, VAT2, solute carrier family 18 (vesicular monoamine), member 2, MGC120477, MGC120478, MGC26538

Gene ID: 6571, 214084, 25549

NCBI Accession: NP_003045