LIG1 antibody (Internal Region)

Catalog No. ABIN571018

This Goat Polyclonal antibody specifically detects LIG1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for LIG1 antibody (Internal Region) (ABIN571018)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LIG1 Antibody

Binding Specificity: Internal Region

Purpose: LIG1 / DNA ligase I

Sequence: RVREDKQPEQ ATTS

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 125 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102 kDa according to NP_000225.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1

Background: LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I

Gene ID: 3978, 81513

NCBI Accession: NP_000225

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA