IL2RG antibody (PE)

Catalog No. ABIN5710229

The Rat Monoclonal anti-IL2RG antibody has been validated for FACS. It is suitable to detect IL2RG in samples from Human and Dog.

Quick Overview for IL2RG antibody (PE) (ABIN5710229)

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Reactivity: Human, Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2RG antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: TUGh4

Product Details anti-IL2RG Antibody (PE)

Purpose: Anti-Hu CD132 PE

Specificity: The rat monoclonal antibody TUGh4 recognizes an extracellular epitope of CD132 (the common gamma chain), a 65-70 kDa type I transmembrane glycoprotein broadly expressed by most leukocytes.

Cross-Reactivity (Details): Human, Canine (Dog)

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD132-transfected cell line

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for IL2RG

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Alternative Name: CD132

Background: Interleukin 2 receptor subunit gamma,CD132 / common gamma chain is an essential component of receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, and it is critical for development of the immune system. Its mutation causes X-linked severe combined immunodeficiency disease (XSCID). CD132 is expressed on lymphocytes, NK cells, monocytes, and granulocytes. Through its cytoplasmic part which containsfour tyrosines and an SH2 domain, CD132 transcuces signal to downstream JAK/STAT pathway.,common gamma chain, IL2RG, SCIDX, IMD4

Gene ID: 3561

UniProt: P31785

Pathways: JAK-STAT Signaling, Growth Factor Binding