Calnexin antibody (Internal Region)

Catalog No. ABIN571025

The Goat Polyclonal anti-Calnexin antibody has been validated for WB, IHC, IF and ELISA. It is suitable to detect Calnexin in samples from Human and Rat.

Quick Overview for Calnexin antibody (Internal Region) (ABIN571025)

Target: Calnexin (CANX)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-Calnexin Antibody

Binding Specificity: Internal Region

Purpose: Calnexin

Sequence: SKTPELNLDQ FHDKT

Specificity: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 6 μg/mL.

Western Blot: Approx 90-100 kDa band observed in Human Cerebellum and Rat Brain lysates and in lysates of cell lines LNCaP and U251 (calculated MW of 67.6 kDa according to Human NP_001737.1 and 67.3 kDa according to Rat NP_742005.1). This molecular weight i

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of U251 cells and in the endoplasmic reticulum and plasma membranes of LNCaP cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX

Background: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88

Gene ID: 821, 12330, 29144

NCBI Accession: NP_001737

Pathways: MAPK Signaling, Thyroid Hormone Synthesis