HMGA1 antibody (AA 9-21)

Catalog No. ABIN571040

The Goat Polyclonal anti-HMGA1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect HMGA1 in samples from Human.

Quick Overview for HMGA1 antibody (AA 9-21) (ABIN571040)

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-HMGA1 Antibody

Binding Specificity: AA 9-21

Purpose: HMGA1 (aa9-21)

Sequence: SQPLASKQEK DGT

Specificity: This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1, NP_002122.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 24 kDa band observed in Human Pancreas (calculated MW of 11.7 kDa/10.7 kDa according to NP_665906.1, NP_002122.1). This molecular weight is routinely observed by other sources. This band was successfully blocked by incubation with the

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Alternative Name: HMGA1

Background: HMGA1, high mobility group AT-hook 1, HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854, OTTHUMP00000016222, OTTHUMP00000016223, OTTHUMP00000016224, OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chrom

Gene ID: 3159, 15361, 117062

NCBI Accession: NP_665906, NP_002122

Pathways: Nuclear Hormone Receptor Binding, Autophagy