NEFM antibody (Internal Region)

Catalog No. ABIN571056

This anti-NEFM antibody is a Goat Polyclonal antibody detecting NEFM in WB, IHC and ELISA. Suitable for Human and Mouse.

Quick Overview for NEFM antibody (Internal Region) (ABIN571056)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NEFM Antibody

Binding Specificity: Internal Region

Purpose: NEFM / NF-M

Sequence: QASHITVERK DYLK

Specificity: This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2, NP_001099011.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 170-200 kDa band observed in Mouse Brain lysates (calculated MW of 102 kDa according to NP_005373.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: NEFM

Background: NEFM, neurofilament, medium polypeptide, NEF3, NF-M, NFM, neurofilament triplet M protein, neurofilament, medium polypeptide 150 kDa, neurofilament-3 (150 kD medium)

Gene ID: 4741, 18040, 24588

NCBI Accession: NP_005373

Pathways: Brown Fat Cell Differentiation