IKZF1 antibody (Internal Region)

Catalog No. ABIN571070

The Goat Polyclonal anti-IKZF1 antibody has been validated for ELISA and IHC. It is suitable to detect IKZF1 in samples from Human.

Quick Overview for IKZF1 antibody (Internal Region) (ABIN571070)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-IKZF1 Antibody

Binding Specificity: Internal Region

Purpose: IKAROS / IKZF1

Sequence: DTPYDSSASY EKEN

Specificity: This antibody is expected to recognise all isoforms NP_001278766.1,NP_001278767.1,NP_001207696.1,NP_001207697.1, NP_001278768.1, NP_001207699.1, NP_001207700.1

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue. Recommended concentration: 2.5-3.8 μg/mL.

Western Blot: Preliminary testing showed a band at approx. 60 kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1 μg/mL antibody staining (calculated MW of 57.5 kDa according to NP_006051.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: IKZF1

Background: IKZF1, IKAROS family zinc finger 1 (Ikaros), Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1, CLL-associated antigen KW-6, Ikaros (zinc finger protein), zinc finger protein, subfamily 1A, 1 (Ikaros), lymphoid transcription factor LyF-1

Gene ID: 10320

NCBI Accession: NP_006051

Pathways: Production of Molecular Mediator of Immune Response