PAX4 antibody (Internal Region)

Catalog No. ABIN571093

This Goat Polyclonal antibody specifically detects PAX4 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PAX4 antibody (Internal Region) (ABIN571093)

Target: PAX4 (Paired Box 4 (PAX4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PAX4 Antibody

Binding Specificity: Internal Region

Purpose: PAX4

Sequence: GKLATATSLP EDTVR

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GKLATATSLPEDTVR, from the internal region of the protein sequence according to NP_006184.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1 kDa according to Human NP_006184.2 and 38.0 kDa according to Mouse NP_035168.1). Recommended concentration: 0.2-0.6 μg

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX4

Target: PAX4 (Paired Box 4 (PAX4))

Alternative Name: PAX4

Background: PAX4, paired box 4, KPD, MGC129960, MODY9, paired box gene 4, paired domain gene 4

Gene ID: 5078, 18506, 83630

NCBI Accession: NP_006184