GLUD1 / GLUD2 (Internal Region) antibody

Catalog No. ABIN571123

This Goat Polyclonal antibody specifically detects GLUD1 / GLUD2 in ELISA, WB, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GLUD1 / GLUD2 (Internal Region) antibody (ABIN571123)

Target: GLUD1 / GLUD2

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Grade: Verified

Product Details

Binding Specificity: Internal Region

Purpose: Glutamate Dehydrogenase

Sequence: ESEEQKRNRV RGILR

Specificity: This antibody is expected to recognize both reported isoforms (NP_005262.1, NP_036216.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1, NP_036216.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4 kDa according to Human NP_005262.1, of 61.3 kDa according to Mouse NP_032159.1 and of 61.4 kDa acco

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: GLUD1 / GLUD2

Background: GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003, glutamate dehydrogenase (NAD(P)+), GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1

Gene ID: 2746, 2747, 14661

NCBI Accession: NP_005262, NP_036216