MECOM antibody (Internal Region)

Catalog No. ABIN571139

The Goat Polyclonal anti-MECOM antibody is suitable to detect MECOM in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MECOM antibody (Internal Region) (ABIN571139)

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MECOM Antibody

Binding Specificity: Internal Region

Purpose: EVI1 / AML1

Sequence: DKESLHSTSH

Specificity: This antibody is expected to recognize all reported isoforms (NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKESLHSTSH, from the internal region of the protein sequence according to NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 170 kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126 kDa according to NP_001098547.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: MECOM

Background: MECOM, MDS1 and EVI1 complex locus, AML1-EVI-1, EVI1, MDS1, MDS1-EVI1, MGC163392, MGC97004, PRDM3, AML1-EVI-1 fusion protein, ecotropic viral integration site 1, myelodysplasia syndrome 1, myelodysplasia syndrome-associated protein 1, oncogene EVI1, zinc

Gene ID: 2122, 14013

NCBI Accession: NP_001098547, NP_005232, NP_004982, NP_001157471, NP_001157472