SPON2 antibody (Internal Region)

Catalog No. ABIN571150

This Goat Polyclonal antibody specifically detects SPON2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SPON2 antibody (Internal Region) (ABIN571150)

Target: SPON2 (Spondin 2 (SPON2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPON2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Recent

Product Details anti-SPON2 Antibody

Binding Specificity: Internal Region

Purpose: Spondin 2 / Mindin

Sequence: RKNQYVSNGL RDFA

Specificity: Reported variants NP_036577.1 and NP_001121797.1 represent identical protein.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RKNQYVSNGLRDFA, from the internal region of the protein sequence according to NP_036577.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in preliminary testing of Human and Mouse Prostate lysate after 1 μg/mL antibody staining (calculated MW of 35.8 kDa according to Human NP_036577.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPON2

Target: SPON2 (Spondin 2 (SPON2))

Alternative Name: SPON2

Background: SPON2, spondin 2, extracellular matrix protein, DIL-1, DIL1, M-spondin, Mindin

Gene ID: 10417

NCBI Accession: NP_036577