SPON2 antibody (Internal Region)

Catalog No. ABIN940566

Product Details anti-SPON2 Antibody

Target: SPON2 (Spondin 2 (SPON2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SPON2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: SPON2 (301-315)

Sequence: RTRYVRVQPA NNGSP

Specificity: Reported variants represent identical protein: NP_036577.1, NP_001121797.1, NP_001185950.1

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RTRYVRVQPANNGSP, from the internal region (near C Terminus) of the protein sequence according to NP_036577.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35-36 kDa band observed in Human, Mouse and Rat Kidney lysates, and in preliminary testing of A549 cell lysates (calculated MW of 35.8 kDa according to Human NP_036577.2,and 36.0 kDa according to Mouse NP_598664.3 and Rat NP_612542.1).

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPON2

Target: SPON2 (Spondin 2 (SPON2))

Alternative Name: SPON2 (SPON2 Products)

Synonyms: DIL-1 antibody, DIL1 antibody, M-SPONDIN antibody, MINDIN antibody, 2310045I24Rik antibody, AI504350 antibody, M-spondin antibody, Mindin antibody, Mspondin antibody, etID309958.14 antibody, mindin2 antibody, zgc:100756 antibody, mindin1 antibody, spondin 2 antibody, spondin 2, extracellular matrix protein antibody, spondin 2, extracellular matrix protein S homeolog antibody, spondin 2b, extracellular matrix protein antibody, spondin 2a, extracellular matrix protein antibody, SPON2 antibody, Spon2 antibody, spon2.S antibody, spon2b antibody, spon2a antibody

Background: SPON2, spondin 2, extracellular matrix protein, DIL-1, DIL1, DKFZp686G21139, FLJ16313, FLJ22401, FLJ34460, M-SPONDIN, MINDIN, differentially expressed in cancerous and non-cancerous lung cells 1, spondin-2

Gene ID: 10417, 100689, 171569

NCBI Accession: NP_036577