Cullin 2 antibody (C-Term)

Catalog No. ABIN571170

This anti-Cullin 2 antibody is a Goat Polyclonal antibody detecting Cullin 2 in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for Cullin 2 antibody (C-Term) (ABIN571170)

Target: Cullin 2 (CUL2)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Cullin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Cullin 2 Antibody

Binding Specificity: C-Term

Purpose: CUL2

Sequence: QYIERSQASA DE

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QYIERSQASADE, from the C Terminus of the protein sequence according to NP_003582.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0 kDa according to Human NP_003582.2 and of 86.9 kDa according to Mouse NP_083678.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cullin 2

Target: Cullin 2 (CUL2)

Alternative Name: CUL2

Background: CUL2, cullin 2, MGC131970, OTTHUMP00000019437

Gene ID: 8453, 71745, 361258

NCBI Accession: NP_003582

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome