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TNFRSF4 antibody (FITC)

Name: TNFRSF4 antibody (FITC)
Brand: antibodies-online
SKU: ABIN5711930
Price: 503.21 USD
Availability: InStock

TNFRSF4 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal Ber-ACT35 FITC

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μL reagent per milion cells in 100 μL of cell suspension). (TNFRSF4 antibody (FITC))

FACS

Flow cytometry multicolor surface staining of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μL reagent per milion cells in 100 μL of cell suspension) and anti-human CD25 (MEM-181) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (TNFRSF4 antibody (FITC))

FACS

Separation of human CD134 positive CD25 positive cells (red-filled) from CD134 negative CD25 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD134 (Ber-ACT35) FITC antibody (4 μL reagent per milion cells in 100 μL of cell suspension). (TNFRSF4 antibody (FITC))

FACS

3 images

Catalog No. ABIN5711930

Datasheet as PDF

Target See all TNFRSF4 Antibodies

TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Reactivity
72

43

32

4

3

3

3

2

2

1

1
Human, Non-Human Primate
Host
63

32

6

2

1
Mouse
Compatible Secondaries
Clonality
59

44

1
Monoclonal
Conjugate
56

9

8

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TNFRSF4 antibody is conjugated to FITC
Application
55

40

33

15

14

13

12

11

10

6

4

3

3

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Purpose

Anti-Hu CD134 FITC

Specificity

The mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes an extracellular epitope of CD134 (TNFRSF4, OX40), an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.

Cross-Reactivity (Details)

Human, Non-Human Primates

Purification

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

HTLV 1-transformed HUT-102 cells

Clone

Ber-ACT35

Isotype

IgG1 kappa

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TNFRSF4 Reactivity: Human FACS, ELISA, IHC Host: Mouse Monoclonal 3G5G7 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) (AA 59-205) antibody
TNFRSF4 Reactivity: Human, Chimpanzee FACS, ELISA, IHC, IF, Coat, StM, ISt Host: Mouse Monoclonal OX40-3108 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) antibody (PE)
TNFRSF4 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal Ber-ACT35 PE

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) antibody (APC)
TNFRSF4 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal Ber-ACT35 APC

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) (AA 29-214) antibody
TNFRSF4 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 2D1B8 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) antibody
TNFRSF4 Reactivity: Human, Non-Human Primate WB, FACS, ELISA, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal Ber-ACT35 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) (AA 101-200) antibody
TNFRSF4 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) antibody
TNFRSF4 Reactivity: Rat FACS, IP, IHC (fro) Host: Mouse Monoclonal OX-40 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) antibody
TNFRSF4 Reactivity: Human, Cat WB, FACS Host: Mouse Monoclonal 7D6 unconjugated

anti-Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) (AA 29-214) antibody
TNFRSF4 Reactivity: Human ELISA Host: Mouse Monoclonal 7B5E10 unconjugated

Application Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Restrictions

For Research Use only
Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Storage

4 °C

Storage Comment

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name

CD134 (TNFRSF4 Products)

Background

TNF receptor superfamily member 4,CD134 (TNFRSF4, also known as OX40) is a type I transmembrane glycoprotein of TNF/NGF receptor family expressed on activated T cells, fibroblasts, and hematopoietic precursors. Binding to its ligand (OX40L, TNFSF4) on antigen presenting cells gives to the T cell costimulatory signal, and this interaction results also in B cell proliferation and influences T cell memory pool. CD134 is upregulated at sites of inflammation, especially in case of multiple sclerosis and psoriatic lesions.,TNFRSF4, OX40, ACT35, IMD16, TXGP1L

Gene ID

7293

UniProt

P43489

Pathways

Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints