Septin 4 antibody (AA 33-44)

Catalog No. ABIN571249

This Goat Polyclonal antibody specifically detects Septin 4 in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Septin 4 antibody (AA 33-44) (ABIN571249)

Target: Septin 4 (SEPT4)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Septin 4 Antibody

Binding Specificity: AA 33-44

Purpose: SEPT4 (aa33-44)

Sequence: ELSKFVKDFS GN

Specificity: This antibody is expected to recognize all reported isoforms (NP_004565.1, NP_536340.1, NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ELSKFVKDFSGN

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55 kDa in Human Cerebellum lysates, and approx. 50 kDa in Mouse Brain lysates (calculated MW of 53.0 kDa according to Human NP_536341.1 and 49.4kD

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 4

Target: Septin 4 (SEPT4)

Alternative Name: SEPT4

Background: SEPT4, septin 4, ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2, hucep-7, cell division control-related protein 2, cerebral protein 7, peanut-like 2, septin-M

Gene ID: 5414, 18952

NCBI Accession: NP_004565, NP_536340, NP_536341, NP_001185642