IDH1 antibody (AA 65-77)

Catalog No. ABIN571277

The Goat Polyclonal anti-IDH1 antibody has been validated for WB and ELISA. It is suitable to detect IDH1 in samples from Human, Mouse and Rat.

Quick Overview for IDH1 antibody (AA 65-77) (ABIN571277)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IDH1 Antibody

Binding Specificity: AA 65-77

Purpose: IDH1 (aa65-77)

Sequence: KKHNVGVKCA TIT

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: KKHNVGVKCATIT

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40+45 kDa bands observed in lysates of cell line HepG2 and 40 kDa in Mouse and Rat Liver lysates (calculated MW of 46.7 kDa according to NP_005887.2). The observed 40 kDa is consistent with observaton with different antibodies from othe

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background: IDH1, isocitrate dehydrogenase 1 (NADP+), soluble, IDCD, IDH, IDP, IDPC, PICD, NADP+-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, OTTHUMP00000206464, OTTHUMP00000206465, OTTHUMP00

Gene ID: 3417, 15926, 24479

NCBI Accession: NP_005887

Pathways: Warburg Effect