RUFY1 antibody (Internal Region)

Catalog No. ABIN571280

The Goat Polyclonal anti-RUFY1 antibody is suitable to detect RUFY1 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for RUFY1 antibody (Internal Region) (ABIN571280)

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RUFY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RUFY1 Antibody

Binding Specificity: Internal Region

Purpose: RUFY1

Sequence: QKNEAITSFE GKT

Specificity: This antibody is expected to recognize both reported isoforms (NP_079434.3, NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QKNEAITSFEGKT

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 85 kDa band observed in lysates of cell line HeLa (calculated MW of 79.8 kDa according to NP_079434.3). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RUFY1

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Alternative Name: RUFY1

Background: RUFY1, RUN and FYVE domain containing 1, FLJ22251, RABIP4, ZFYVE12, FYVE-finger protein EIP1, OTTHUMP00000223250, RUN and FYVE domain-containing 1, RUN and FYVE domain-containing protein 1, la-binding protein 1, rab4-interacting protein, zinc finger FYVE

Gene ID: 80230, 216724, 360521

NCBI Accession: NP_079434, NP_001035541