His Tag antibody

Catalog No. ABIN571898

The Sheep Polyclonal anti-His Tag antibody has been validated for WB and IHC. It is suitable to detect His Tag in samples from .

Quick Overview for His Tag antibody (ABIN571898)

Target: His Tag

Reactivity: Please inquire

Host: Sheep

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Specificity: Specific for His tag (HHHHHH).

Immunogen: Synthetic hexa His {HHHHHH} conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: A tag commonly fused with the protein of interest and expressed as a single chain. The expressed recombinant protein can then be conveniently purified on Ni-column. Also known as: His tag, Histidin, Hexa His tag, 6x His tag.