HMGB1 antibody (AA 1-91)

Catalog No. ABIN574269

Product Details anti-HMGB1 Antibody

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Binding Specificity: AA 1-91

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Purified from ascites by Protein A

Immunogen: HMGB1 (NP_002119, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Clone: 2F6

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IF (10 μg/mL), IHC, IHC-P (5 μg/mL), WB

Usage: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 μg/mL) - positive control HeLa cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMG1 / HMGB1 (HMGB1 Products)

Background: Name/Gene ID: HMGB1

Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1

Gene ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome