Calnexin antibody (AA 204-218)

Catalog No. ABIN574328

The Goat Polyclonal anti-Calnexin antibody is suitable to detect Calnexin in samples from Human, Mouse, Dog, Cow, Monkey, Rabbit, Pig, Chicken, Horse and Bat. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for Calnexin antibody (AA 204-218) (ABIN574328)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Dog, Cow, Monkey, Rabbit, Pig, Chicken, Horse, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calnexin Antibody

Binding Specificity: AA 204-218

Specificity: Human CANX / Calnexin. Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Chicken (100%) Rat, Hamster, Turkey, Platypus (93%) Marmoset, Mouse (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SKTPELNLDQFHDKT from an internal region (near N-terminus) of human CANX / Calnexin (NP_001737.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Chicken (100%), Rat, Hamster, Turkey, Platypus (93%), Marmoset, Mouse (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Western Blot: Approx. 100kD band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kD according to Human NP_001737.1 and 67.2kD according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7,271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX / Calnexin

Background: Name/Gene ID: CANX

Synonyms: CANX, CNX, Calnexin, IP90, p90

Gene ID: 821

NCBI Accession: NP_001737

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis