SHC1 antibody (AA 171-280)

Catalog No. ABIN574341

The Mouse Monoclonal anti-SHC1 antibody (Clone 3F4) (ABIN574341) specifically detects SHC1 in WB, IHC, ELISA, IF, IHC (p) and PLA. The antibody is reactive with Human samples.

Quick Overview for SHC1 antibody (AA 171-280) (ABIN574341)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Clone: 3F4

Product Details anti-SHC1 Antibody

Binding Specificity: AA 171-280

Purification: Purified from ascites by Protein A

Immunogen: SHC1 (AAH33925, 171 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG kappa

Application Details

Application Notes: Approved: ELISA, IF (10 μg/mL), IHC, IHC-P (1 μg/mL), PLA, WB

Usage: Western Blot (Cell lysate) - positive control A-431. Immunofluorescence (10 μg/mL) - positive control A-431 cells. Sandwich ELISA (Recombinant protein). In situ Proximity Ligation Assay (Cell).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: p66 / SHC

Background: Name/Gene ID: SHC1

Synonyms: SHC1, p46 shc, p52 shc, SH2 domain protein C1, p66, SHCA, SHC, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A

Gene ID: 6464

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2