CD4 antibody (PE)

Catalog No. ABIN576507

This anti-CD4 antibody is a Rat Monoclonal antibody detecting CD4 in FACS. Suitable for Mouse.

Quick Overview for CD4 antibody (PE) (ABIN576507)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-CD4 Antibody (PE)

Specificity: Detects mouse CD4, a 55 kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. The RM4-5 antibody is useful for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

Purification: Protein G purified

Immunogen: BALB/c mouse thymocytes.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule

Gene ID: 920

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events