CD80 antibody (PE)

Catalog No. ABIN576900

This anti-CD80 antibody is a Rat Monoclonal antibody detecting CD80 in FACS, IHC and IHC (fro). Suitable for Mouse.

Quick Overview for CD80 antibody (PE) (ABIN576900)

Target: CD80

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RM80

Product Details anti-CD80 Antibody (PE)

Specificity: Recognizes mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

Purification: Affinity purified

Immunogen: BCL1 cells expressing CD80.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1), IHC, IHC-Fr

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: Name/Gene ID: CD80

Synonyms: CD80, Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CD80 antigen, LAB7, CD80 Molecule, CTLA-4 counter-receptor B7.1

Gene ID: 941

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints