DNMT1 antibody

Catalog No. ABIN594698

The Rabbit Monoclonal anti-DNMT1 antibody has been validated for WB and IF. It is suitable to detect DNMT1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for DNMT1 antibody (ABIN594698)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-DNMT1 Antibody

Specificity: Recognizes endogenous levels of human total DNMT1 protein. Species cross-reactivity: mouse, rat, monkey. Sequence Homology: bovine.

Purification: Purified

Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence of human DNMT1 protein.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IF, WB (1:1000)

Usage: Suitable for use in Western Blot and Immunofluorescence (IC). Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 %. Tween-20 at 4 °C with gentle shaking, overnight. Immunofluorescence (IC): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. For long-term storage and to avoid freeze-thaw cycles, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT / DNMT1

Background: Name/Gene ID: DNMT1
Family: C5-methyltransferase

Synonyms: DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection