POLR2A/RPB1 antibody (Ser1619)

Catalog No. ABIN5959399

The Rabbit Polyclonal anti-POLR2A/RPB1 antibody (ABIN5959399) specifically detects POLR2A/RPB1 in WB, IF, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for POLR2A/RPB1 antibody (Ser1619) (ABIN5959399)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: Ser1619

Specificity: DNA directed RNA polymerase II A,DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit,DNA-directed RNA polymerase II subunit A,DNA-directed RNA polymerase II subunit RPB1,DNA-directed RNA polymerase III largest subunit,hRPB220,hsRPB1,POLR2,Polr2a,POLRA,Polymerase (RNA) II (DNA directed) polypeptide A 220  kDa,Polymerase (RNA) II (DNA directed) polypeptide A,RNA polymerase II subunit B1,RNA-directed RNA polymerase II subunit RPB1,RPB1,RPB1,RPBh1,RpIILS,RPO2,RPOL2

Purification: Affinity purification

Immunogen: Synthesized peptide derived from human Rpb1 around the non-phosphorylation site of Ser1619.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: Rpb1

Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight: 217kDa

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways