GFP Tag antibody

Catalog No. ABIN5961049

The Mouse Monoclonal anti-GFP Tag antibody (Clone 6G4) (ABIN5961049) specifically detects GFP Tag in WB. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP Tag antibody (ABIN5961049)

Target: GFP Tag

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6G4

Product Details anti-GFP Tag Antibody

Specificity: GFP,Green fluorescent protein

Purification: Protein A purification

Immunogen: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: WB 1:2000-10000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for GFP Tag

Target: GFP Tag

Alternative Name: GFP-Tag

Target Type: Tag

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9  kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).