TNF alpha antibody (Biotin)

Catalog No. ABIN598279

This Mouse Monoclonal antibody specifically detects TNF alpha in ELISA and FACS. It exhibits reactivity toward Cow and Sheep.

Quick Overview for TNF alpha antibody (Biotin) (ABIN598279)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-TNF alpha Antibody (Biotin)

Specificity: Recognizes bovine TNF, alpha. Species cross-reactivity: sheep.

Purification: Protein G purified

Immunogen: Recombinant bovine TNF alpha.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1 - 5 μg/mL), Flo (1:10 - 1:100)

Usage: Suitable for use in ELISA and Suitable for use in Flow cytometry: 1:10-1:100, 10 μL labels 10^6 cells in 100 μL. ELISA: 1-5 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome