PPARA antibody (AA 1-18)

Catalog No. ABIN600113

The Rabbit Polyclonal anti-PPARA antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect PPARA in samples from Human, Mouse and Monkey.

Quick Overview for PPARA antibody (AA 1-18) (ABIN600113)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPARA Antibody

Binding Specificity: AA 1-18

Specificity: Recognizes mouse PPAR alpha. Species cross-reactivity: Human, monkey, Chimpanzee.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa1-18 of mouse PPAR alpha.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:8000), IHC, IHC-P (20 μg/mL), WB (1:500)

Usage: Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. ELISA: 1:8000. Western Blot: 1:500. Immunohistochemistry (paraffin): 20 μg/mL. Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR alpha

Background: Name/Gene ID: PPARA
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARA, PPAR Alpha, PPARalpha, PPAR-alpha, NR1C1, HPPAR, PPAR

Gene ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C