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CD25 antibody

This anti-CD25 antibody is a Rat Monoclonal antibody detecting CD25 in FACS, IHC, IP and IHC (fro). Suitable for Mouse.
Catalog No. ABIN601656

Quick Overview for CD25 antibody (ABIN601656)

Target

See all CD25 (IL2RA) Antibodies
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity

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Mouse

Host

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Rat

Clonality

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Monoclonal

Conjugate

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This CD25 antibody is un-conjugated

Application

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Specificity

    Recognizes the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Inhibits IL-2 binding and IL-2 dependent proliferation.

    Purification

    Protein G purified

    Immunogen

    B6.1 CTL cell line.

    Type of Immunogen: Cells

    Isotype

    IgG1
  • Application Notes

    Approved: Flo, IHC, IHC-Fr, IP

    Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Flow Cytometry: 10 μL labels 10^6 cells in 100 μL.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing

    Storage

    4 °C,-20 °C

    Storage Comment

    May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
  • Target

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    Alternative Name

    IL2RA / CD25

    Background

    Name/Gene ID: IL2RA
    Family: Interleukin

    Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

    Gene ID

    3559

    UniProt

    P01589

    Pathways

    JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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