IRS1 antibody (pSer302)

Catalog No. ABIN601681

This Mouse Monoclonal antibody specifically detects IRS1 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IRS1 antibody (pSer302) (ABIN601681)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-IRS1 Antibody

Binding Specificity: pSer302

Specificity: Recognizes IRS1 phosphorylated at Ser302 (mouse) and Ser307 (human). Species cross-reactivity: rat. Species sequence homology: monkey, bovine, canine, porcine. Predicted to cross-react with many other species based on 100 % sequence homology with immunogen.

Purification: Protein G purified

Immunogen: Synthetic linear peptide corresponding to amino acids at and around phosphorylated Ser302 of mouse IRS1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF, IHC, IP (1:1000), WB (1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:1000.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 / IRS-1

Background: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process