PDPK1 antibody (pSer241)

Catalog No. ABIN602119

This Rabbit Polyclonal antibody specifically detects PDPK1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDPK1 antibody (pSer241) (ABIN602119)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PDPK1 Antibody

Binding Specificity: pSer241

Specificity: Recognizes human PDK1 when phosphorylated at serine 241. Species cross-Reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:200), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Immunofluorescence:1:100-1:200. Western Blot:1:500-1:1000. Immunohistochemistry:1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1 / PDPK1

Background: Name/Gene ID: PDPK1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: PDPK1, HPDK1, PkB kinase like gene 1, PkB-like 1, Pdk-1, PkB kinase, PRO0461

Gene ID: 5170

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals