RAGE antibody (Cy3)

Catalog No. ABIN604898

The Mouse Monoclonal anti-RAGE antibody is suitable to detect RAGE in samples from Human and Cow. It has been validated for WB, IHC, ELISA, ICC and IHC (p).

Quick Overview for RAGE antibody (Cy3) (ABIN604898)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAGE antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DD-A11

Product Details anti-RAGE Antibody (Cy3)

Specificity: Recognizes both natural and recombinant RAGE. Species cross-reactivity: Human and bovine.

Purification: Protein A purified

Immunogen: Recombinant truncated extracellular portion of human RAGE expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P (10 μg/mL), WB (1:250)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Western Blot: 1:250. ~48kD band in mouse brain lysates. Immunohistochemistry: Paraffin embedded tissue sections. Immunocytochemistry: Cells fixed with ice cold acetone using a secondary antibody labeled with Cy3. ELISA: Direct. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: AGER / RAGE

Background: Name/Gene ID: AGER

Synonyms: AGER, RAGE, RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta

Gene ID: 177

UniProt: Q15109

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins