CD11b antibody (AA 500-600) (HRP)
Quick Overview for CD11b antibody (AA 500-600) (HRP) (ABIN608932)
Target
See all CD11b (ITGAM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 500-600
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Specificity
- Recognizes CD11b/c. Using macrophage lysates, a doublet is seen ~95 and 103kD. A weaker doublet is also seen ~130 and 160kD. Species cross-reactivity: human and mouse. Cellular Localization: monocytes and granulocytes.
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Purification
- Immunoaffinity purified
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Immunogen
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A synthetic peptide made to an internal region (aa500-600) of the mouse CD11 (b/c) protein.
Type of Immunogen: Synthetic peptide
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Application Notes
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Approved: IHC, IHC-P (5 - 10 μg/mL), WB (2 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 2 μg/mL. Immunohistochemistry (regular/paraffin): 5-10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide. Labeled, HRP
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Do not freeze! Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates
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Storage
- 4 °C
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Storage Comment
- Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
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- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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Alternative Name
- CD11b + C
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Background
- This antibody detects: CD11b + c
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Pathways
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Target
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