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CD11b antibody (AA 500-600) (HRP)

This Rabbit Polyclonal antibody specifically detects CD11b in IHC, WB and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN608932

Quick Overview for CD11b antibody (AA 500-600) (HRP) (ABIN608932)

Target

See all CD11b (ITGAM) Antibodies
CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity

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Human, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CD11b antibody is conjugated to HRP

Application

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Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 500-600

    Specificity

    Recognizes CD11b/c. Using macrophage lysates, a doublet is seen ~95 and 103kD. A weaker doublet is also seen ~130 and 160kD. Species cross-reactivity: human and mouse. Cellular Localization: monocytes and granulocytes.

    Purification

    Immunoaffinity purified

    Immunogen

    A synthetic peptide made to an internal region (aa500-600) of the mouse CD11 (b/c) protein.

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: IHC, IHC-P (5 - 10 μg/mL), WB (2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 2 μg/mL. Immunohistochemistry (regular/paraffin): 5-10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide. Labeled, HRP

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze! Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
  • Target

    CD11b (ITGAM) (Integrin alpha M (ITGAM))

    Alternative Name

    CD11b + C

    Background

    This antibody detects: CD11b + c

    Pathways

    Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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