Cytokeratin 7 antibody

Catalog No. ABIN609493

This Mouse Monoclonal antibody specifically detects Cytokeratin 7 in WB, IHC, IHC (p) and IP. It exhibits reactivity toward Human, Cow and Pig.

Quick Overview for Cytokeratin 7 antibody (ABIN609493)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Cytokeratin 7 Antibody

Specificity: Recognizes a unique epitope shared by the intermediate filament keratins, of human cytokeratin 7 and cytokeratin 17, a 54kD type II basic cytokeratin, and 46kD type I acidic cytokeratin respectively. Species cross-reactivity: bovine, porcine. Does not react with mouse, rabbit, rat.

Purification: Protein A purified

Immunogen: HeLa cell cytoskeleton preparation.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (20 - 30 μg/mL), IP, WB (2 - 3 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunohistochemistry (paraffin), and Western Blot. Immunohistochemistry (paraffin): 20-30 μg/mL. Western Blot: 2-3 μg/mL. Requires protein digestion pre-treatment of paraffin sections using pepsin prior to staining.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: KRT7 / CK7 / Cytokeratin 7

Background: Name/Gene ID: KRT7

Synonyms: KRT7, CK-7, Cytokeratin 7, CK7, K2C7, K7, Keratin 7, Keratin-7, SCL, Type II mesothelial keratin K7, Cytokeratin-7, Sarcolectin, Type-II keratin Kb7

Gene ID: 3855