CD25 antibody

Catalog No. ABIN610001

The Mouse Monoclonal anti-CD25 antibody has been validated for FACS and IHC. It is suitable to detect CD25 in samples from Human. There are 4+ publications available.

Quick Overview for CD25 antibody (ABIN610001)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 7G7B6

Product Details anti-CD25 Antibody

Specificity: Epitope B of the IL-2 alpha receptor of about 55 kd.

Purification: Protein A purified

Immunogen: Stimulated PBMC.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC

Usage: The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride

Preservative: Azide free

Handling Advice: Do not freeze. Open under aseptic conditions.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Open under aseptic conditions.

References for anti-CD25 antibody (ABIN610001)

Dhillon, Pollock, Steen, Shaw, Mischak, Kolch: "Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3237-46, (2002) (PubMed).

Spaargaren, Bos: "Rab5 induces Rac-independent lamellipodia formation and cell migration." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3239-50, (1999) (PubMed).

Bourne, Sanders, McCormick: "The GTPase superfamily: a conserved switch for diverse cell functions." in: Nature, Vol. 348, Issue 6297, pp. 125-32, (1990) (PubMed).

Gibbs, Marshall: "The ras oncogene--an important regulatory element in lower eucaryotic organisms." in: Microbiological reviews, Vol. 53, Issue 2, pp. 171-85, (1989) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2RA / CD25

Background: Name/Gene ID: IL2RA
Family: Interleukin

Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation