ITGA4 antibody (FITC)

Catalog No. ABIN610120

This Mouse Monoclonal antibody specifically detects ITGA4 in FACS, IHC and Func. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for ITGA4 antibody (FITC) (ABIN610120)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func)

Clone: BU49

Product Details anti-ITGA4 Antibody (FITC)

Specificity: CD49d molecule of about 145 kd.

Purification: Protein A purified

Immunogen: Human peripheral myeloma cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Func, IHC

Usage: Function: Blocks VLA-4 binding to VCAM-1.For Flow cytometry use 1.6 μL of concentrated stock per 5 x 10e5-cell test The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5 % Glycerol, 0.2 % BSA, 0.04 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

References for anti-ITGA4 antibody (FITC) (ABIN610120)

Lemmink, Tuyt, Knol, Krikke, Vellenga: "Identification of LIL-STAT in monocytic leukemia cells and monocytes after stimulation with interleukin-6 or interferon gamma." in: Blood, Vol. 98, Issue 13, pp. 3849-52, (2001) (PubMed).

Chang, Peng, Fu: "Interleukin-4 mediates cell growth inhibition through activation of Stat1." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10212-7, (2000) (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: ITGA4 / VLA-4 / CD49d

Background: Name/Gene ID: ITGA4
Family: Integrin

Synonyms: ITGA4, 269C wild type, CD49D, IA4, Integrin alpha-4, Integrin alpha 4, Integrin alpha-4 subunit, Integrin alpha-IV, VLA-4, VLA-4 subunit alpha, CD49d antigen

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex