ATP5F1 antibody (AA 1-245)

Catalog No. ABIN6137331

There are 2+ publications for this product available. The Rabbit Polyclonal anti-ATP5F1 antibody is suitable to detect ATP5F1 in samples from Human. It has been validated for WB and IF.

Quick Overview for ATP5F1 antibody (AA 1-245) (ABIN6137331)

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ATP5F1 Antibody

Binding Specificity: AA 1-245

Sequence: MLSRVVLSAA ATAAPSLKNA AFLGPGVLQA TRTFHTGQPH LVPVPPLPEY GGKVRYGLIP EEFFQFLYPK TGVTGPYVLG TGLILYALSK EIYVISAETF TALSVLGVMV YGIKKYGPFV ADFADKLNEQ KLAQLEEAKQ ASIQHIQNAI DTEKSQQALV QKRHYLFDVQ RNNIAMALEV TYRERLYRVY KEVKNRLDYH ISVQNMMRRK EQEHMINWVE KHVVQSISTQ QEKETIAKCI ADLKL

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human ATP5F1 (NP_001679.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-ATP5F1 antibody (ABIN6137331)

Fan, Long, Yan, Wang, Shi, Bao, Hu, Li, Chen, Zheng, Yan: "Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets." in: The British journal of nutrition, Vol. 117, Issue 9, pp. 1222-1234, (2017) (PubMed).

Hu, Nie, Chen, Xie, Fan, Wang, Long, Yan, Zhong, Yan: "Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1α pathway in porcine intestinal epithelial cells." in: The international journal of biochemistry & cell biology, Vol. 89, pp. 42-56, (2017) (PubMed).

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.,ATP5F1,PIG47,Signal Transduction,Endocrine & Metabolism,Neuroscience,Neurodegenerative Diseases,ATP5F1

Molecular Weight: 28 kDa

Gene ID: 515

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process